The Phospho-Bcl-2-S87 Antibody (CABP1071) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the phosphorylated form of Bcl-2 at serine 87, this antibody allows for the detection and analysis of this important protein modification in various cell types.Bcl-2 is a critical regulator of apoptosis, and phosphorylation at serine 87 has been shown to affect its anti-apoptotic function.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Bcl-2-S87 Antibody
SKU:
CABP1071
Size:
20μL, 100μL
Reactivity:
Human, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ALSP V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
596
RRID
AB_2863943
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using Phospho-Bcl-2-S87 Rabbit pAb (CABP1071) at 1:1000 dilution. C6 cells were treated with Paclitaxel (100 nM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
