The Phospho-BLM T99 Antibody (PACO05014) is a valuable tool for researchers studying the BLM protein, a key player in DNA damage repair processes. This antibody, produced in rabbits, is highly specific to phosphorylated BLM at threonine 99, allowing for precise detection and analysis in Western blot experiments. Its reactivity with human samples makes it an ideal choice for studies in cell biology, genetics, and cancer research.BLM, a member of the RecQ helicase family, plays a crucial role in maintaining genomic stability by resolving DNA structures and promoting accurate DNA replication. Phosphorylation of BLM at threonine 99 is known to regulate its function in DNA repair pathways, making this site of interest for investigating the mechanisms underlying genomic instability and cancer development.
By utilizing the Phospho-BLM T99 Antibody, researchers can further our understanding of the molecular mechanisms involved in DNA repair processes and identify potential targets for therapeutic interventions in diseases characterized by genomic instability, such as cancer. Its high specificity and sensitivity make it a valuable tool for advancing research in the field of DNA damage response and repair.
Synthesized peptide derived from human BLM around the phosphorylation site of T99.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3
UniProt Protein Function:
BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.Protein type: DNA repair, damage; Helicase; DNA replication; EC 3.6.4.12Chromosomal Location of Human Ortholog: 15q26.1Cellular Component: chromosome, telomeric region; cytoplasm; lateral element; nuclear chromosome; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML bodyMolecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; p53 binding; protein binding; single-stranded DNA bindingBiological Process: DNA double-strand break processing; DNA replication; DNA synthesis during DNA repair; double-strand break repair via homologous recombination; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; positive regulation of transcription, DNA-dependent; protein oligomerization; protein sumoylation; regulation of cyclin-dependent protein kinase activity; replication fork processing; response to DNA damage stimulus; response to X-ray; strand displacementDisease: Bloom Syndrome
UniProt Protein Details:
NCBI Summary:
The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008]
