The Phospho-BRD4-T249 Polyclonal Antibody (CABP1515) is a high-quality antibody developed for reliable detection and analysis of target proteins. BRD4 is a key player in regulating gene expression and cell growth, and phosphorylation at threonine 249 is known to be important for its function. This antibody, raised in rabbits, specifically recognizes phosphorylated BRD4 at T249 in human samples and is validated for use in Western blot applications. By targeting the phosphorylated form of BRD4, researchers can gain insight into the regulation of gene expression, cell proliferation, and other critical cellular processes.
This antibody is validated for use in WB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-BRD4-T249 Polyclonal Antibody
SKU:
CABP1515
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Purification Method
Affinity purification
Gene ID
23476
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-BRD4-T249 Rabbit pAb (CABP1515) at 1:1000 dilution. NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
