The Phospho-BTK-Y223 Monoclonal Antibody (CABP1136) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed from rabbit monoclonal cells, exhibits high specificity and sensitivity towards phosphorylated BTK at the Y223 site.BTK is a critical factor in B-cell development and function, making it a significant target for research in immunology and cancer biology. Phosphorylation of BTK at the Y223 site is known to regulate its kinase activity and downstream signaling pathways, making this antibody essential for investigating the functional implications of BTK phosphorylation in various cellular contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-BTK-Y223 Monoclonal Antibody
SKU:
CABP1136
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0307
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
695
RRID
AB_2864004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using Phospho-BTK-Y223 Rabbit mAb (CABP1136) at 1:500 dilution. Raji cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
