The Phospho-Camk2-T286 Monoclonal Antibody (CABP1386) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through monoclonal antibody technology, is highly specific to the phosphorylated form of CaMK2 at threonine 286 and has been validated for use in various applications, including Western blotting and immunofluorescence.CaMK2 is a serine/threonine protein kinase that plays a crucial role in multiple cellular processes, including learning and memory, neuronal development, and synaptic transmission. Phosphorylation of CaMK2 at threonine 286 is known to regulate its kinase activity and subcellular localization, making it a critical event in neuronal signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-Camk2-T286 Monoclonal Antibody
SKU:
CABP1386
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC57709
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
QETV D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2 )/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Several transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method
Affinity purification
Gene ID
816 817 818 815
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain using Phospho-Camk2-T286 Rabbit mAb (CABP1386) at 1:1000 dilution incubated at room temperature for 1.5 hours. Mouse brain treated with CIP (1U/ul) at 37℃ for 1 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
