Description
Phospho-Chk2-T68 Antibody (CABP0590)
The Phospho-Chk2-T68 Antibody (CABP0590) is a high-quality antibody developed for reliable detection and analysis of target proteins. Chek2 is a crucial regulator of cell cycle progression and DNA damage response, and phosphorylation at T68 is known to activate its kinase activity.Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the phosphorylated form of Chek2 at T68, researchers can easily detect and analyze the protein in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | Phospho-Chk2-T68 Antibody |
| SKU: | CABP0590 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | VSTQ E | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425, Phospho-Chk2-T68 |
| Positive Sample: | HeLa treated with UV |
| Cellular Localization: | Nucleus, Nucleus, Pml Body, Nucleoplasm. |
| Calculated MW: | 61kDa |
| Observed MW: | 62kDa |
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
| Purification Method | Affinity purification |
| Gene ID | 11200 |
| RRID | AB_2771000 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from HeLa cells, using Phospho-Chk2-T68 Rabbit pAb (CABP0590). HeLa cells were treated with UV for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. |
