The Phospho-EZR-T567/RDX-T564/MSN-T558 Antibody (CABP1121) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is raised in rabbits and has high reactivity with human samples, making it ideal for Western blot applications.Phosphorylation of EZR, RDX, and MSN is known to be involved in regulating processes such as cell migration, adhesion, and cytoskeletal organization. This antibody specifically targets the phosphorylated forms of these proteins, allowing for the detection and analysis of their activity in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-EZR-T567/RDX-T564/MSN-T558 Antibody
SKU:
CABP1121
Size:
20μL, 100μL
Reactivity:
Human, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YKTL R
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
PC-12 treated with ATP
Calculated MW:
23-71kDa
Observed MW:
75-80kDa
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Purification Method
Affinity purification
Gene ID
7430 4478 5962
RRID
AB_2863990
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-12 cells, using Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb (CABP1121) at 1:1000 dilution. PC-12 cells were treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
