The Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Polyclonal Antibody (CABP1284) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using advanced technology, specifically targets the phosphorylated forms of ezrin at Thr567, radixin at Thr564, and moesin at Thr558.The ERM proteins play essential roles in cell structure and function, including cell adhesion, migration, and membrane-cytoskeleton interactions. Phosphorylation of these proteins at the specified sites is known to modulate their activity and influence cellular processes. The CABP1284 antibody enables the detection and analysis of phosphorylated ERM proteins in various cell types, providing valuable insights into their function and regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
HeLa treated with TNF-α and Calyculin A, A-431 treated with EGF
Cellular Localization:
Actin Cytoskeleton, Apical Plasma Membrane, Basolateral Plasma Membrane, Cell Projection, Ciliary Basal Body, Cortical Cytoskeleton, Cytoplasm, Cytoplasmic Side Of Apical Plasma Membrane, Cytosol, Endosome, Extracellular Exosome, Extracellular Space, Fibrillar Center, Filopodium, Focal Adhesion, Immunological Synapse, Microvillus Membrane, Perinuclear Region Of Cytoplasm, Plasma Membrane, Plasma Membrane Raft, Ruffle Membrane.
Calculated MW:
23-71kDa
Observed MW:
75-80kDa
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Purification Method
Affinity purification
Gene ID
7430 5962 4478
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Rabbit pAb (CABP1284) at 1:1000 dilution. HeLa cells were treated with TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 10s.
