Description
Phospho-FGFR1-Y653/Y654 Polyclonal Antibody (CABP1317)
The Phospho-FGFR1-Y653/Y654 Polyclonal Antibody (CABP1317) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for Western blot applications.FGFR1 is a transmembrane receptor tyrosine kinase that plays a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of FGFR1 at tyrosine residues 653 and 654 is essential for its activation and downstream signaling, making it a key event in various cellular processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
| Product Name: | Phospho-FGFR1-Y653/Y654 Polyclonal Antibody |
| SKU: | CABP1317 |
| Size: | 20μL, 100μL |
| Reactivity: | Mouse |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | IDYY KK | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | CEK, FLG, HH2, OGD, ECCL, FLT2, KAL2, BFGFR, CD331, FGFBR, FLT-2, HBGFR, N-SAM, FGFR-1, HRTFDS, bFGF-R-1, Phospho-FGFR1-Y653/Y654 |
| Positive Sample: | NIH/3T3 |
| Cellular Localization: | Cytoplasmic Vesicle, Cytosol, Extracellular Region, Nucleus, Plasma Membrane. |
| Calculated MW: | 92kDa |
| Observed MW: | 120kDa |
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
| Purification Method | Affinity purification |
| Gene ID | 2260 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using Phospho-FGFR1-Y653/Y654 Rabbit pAb (CABP1317) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
