The FOXO3A Monoclonal Antibody (CAB9270) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high specificity and sensitivity to human samples, making it ideal for use in Western blot applications.FOXO3A is a crucial mediator of cellular responses to stress and is involved in the regulation of longevity and age-related diseases. Dysregulation of FOXO3A has been linked to various health conditions including cancer, neurodegenerative diseases, and cardiovascular disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FOXO3A Monoclonal Antibody
SKU:
CAB9270
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1505
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2
Positive Sample:
HeLa, PC-3, Mouse lung, Mouse brain
Cellular Localization:
Cytoplasm, Nucleus, Cytosol.
Calculated MW:
71kDa
Observed MW:
90kDa
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method
Affinity purification
Gene ID
2309
RRID
AB_2863703
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FOXO3A Rabbit mAb (CAB9270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Western blot analysis of various lysates using FOXO3A Rabbit mAb (CAB9270) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
