The Phospho-GYS1-S641 Monoclonal Antibody (CABP1129) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed for use in Western blot applications and is highly reactive with samples from various species, including human, mouse, and rat.GYS1 is a key enzyme involved in glycogen synthesis, which plays a critical role in regulating energy storage and metabolism. Phosphorylation of GYS1 at serine 641 is known to modulate its activity, impacting glycogen synthesis and glucose homeostasis. Research on this post-translational modification of GYS1 is important for understanding its regulatory mechanisms in metabolic diseases, such as diabetes and glycogen storage disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-GYS1-S641 Monoclonal Antibody
SKU:
CABP1129
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0207
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GSY, GYS, Phospho-GYS1-S641
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
84kDa
Observed MW:
85kDa
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
2997
RRID
AB_2863997
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit mAb (CAB4774) at 1:1000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
