Description
Phospho-HIST1H1D (T146) Antibody (PACO56661)
The Phospho-HIST1H1D (T146) Antibody (PAC05661) is a valuable tool for researchers studying post-translational modifications of the histone protein HIST1H1D at threonine 146. This antibody, raised in rabbits, is highly specific to the phosphorylated form of HIST1H1D and is validated for use in various applications, including Western blotting and immunofluorescence.Histones are essential proteins involved in packaging DNA into chromatin, and their phosphorylation plays a crucial role in regulating gene expression and chromatin structure. The phosphorylation of HIST1H1D at threonine 146 has been linked to various cellular processes, including cell cycle regulation, DNA repair, and transcriptional activation.
By utilizing the Phospho-HIST1H1D (T146) Antibody, researchers can investigate the functional significance of this post-translational modification and its implications in processes like cellular differentiation, development, and disease. This antibody is a valuable tool for studies in epigenetics, cancer biology, and cell signaling pathways, providing insights into the role of histone phosphorylation in gene regulation and cellular function.
| Antibody Name: | Phospho-HIST1H1D (T146) Antibody (PACO56661) |
| Antibody SKU: | PACO56661 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:200-1:2000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence around site of Phospho-Thr (146) derived from Human Histone H1.3 |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: HIST1H1D antibody at 1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa. |
 | Immunofluorescence staining of Hela cells with PACO56661 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Synonyms: | Histone H1.3 (Histone H1c) (Histone H1s-2), HIST1H1D, H1F3 |
| UniProt Protein Function: | H1D: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: nuclear chromatin Molecular Function:chromatin DNA binding |
| NCBI Summary: | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P16402 |
| NCBI GenInfo Identifier: | 121925 |
| NCBI Gene ID: | 3007 |
| NCBI Accession: | P16402.2 |
| UniProt Secondary Accession: | P16402,Q2M2I2, B2R751, |
| UniProt Related Accession: | P16402 |
| Molecular Weight: | 22,350 Da |
| NCBI Full Name: | Histone H1.3 |
| NCBI Synonym Full Names: | histone cluster 1, H1d |
| NCBI Official Symbol: | HIST1H1DÂ Â |
| NCBI Official Synonym Symbols: | H1D; H1.3; H1F3; H1s-2Â Â |
| NCBI Protein Information: | histone H1.3 |
| UniProt Protein Name: | Histone H1.3 |
| UniProt Synonym Protein Names: | Histone H1c; Histone H1s-2 |
| UniProt Gene Name: | HIST1H1DÂ Â |
| UniProt Entry Name: | H13_HUMAN |
