The Phospho-Y721 Polyclonal Antibody (CABP0385) is a crucial tool for researchers studying protein phosphorylation events within cells. This antibody, raised in rabbits, specifically recognizes phosphorylation at the Y721 site on various proteins, allowing for the detection and analysis of phosphorylated targets in Western blot applications. Phosphorylation of proteins at specific sites, such as Y721, plays a crucial role in the regulation of various cellular processes, including cell signaling, growth, and differentiation. By targeting phosphorylation at the Y721 site, this antibody enables researchers to study the dynamics of protein phosphorylation and its impact on cellular function.
This antibody is validated for use in human samples and offers a valuable tool for investigating signaling pathways and protein phosphorylation events in diseases such as cancer, diabetes, and neurodegenerative disorders. By understanding the role of phosphorylation at the Y721 site, researchers can gain insights into potential therapeutic targets and develop strategies for targeting abnormal phosphorylation events in disease states.
Product Name:
Phospho-KIT-Y721 Rabbit Polyclonal Antibody
SKU:
CABP0385
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding Y721 of human KIT
Cell membrane, Cytoplasm, Single-pass type I membrane protein.
Calculated MW:
110kDa
Observed MW:
130kDa
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3815
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CD117/c-Kit-Y721 Rabbit pAb (CABP0385).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
