The KSR1 Antibody (CAB15118) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for Western blot applications, allowing for the detection and analysis of KSR1 protein in various cell types.KSR1 is known for its role in regulating the Ras-MAPK pathway, which is crucial for cell growth, differentiation, and survival. Dysregulation of this pathway is commonly observed in cancer, making KSR1 a potential target for cancer therapy research.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KSR1 Antibody
SKU:
CAB15118
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables 14-3-3 protein binding activity; ATP binding activity; and protein C-terminus binding activity. Involved in positive regulation of MAPK cascade. Located in endoplasmic reticulum and membrane. Part of protein-containing complex. Implicated in breast adenocarcinoma. Biomarker of breast cancer.
Purification Method
Affinity purification
Gene ID
8844
RRID
AB_2762003
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using KSR1 Rabbit pAb (CAB15118) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using KSR1 Rabbit pAb (CAB15118) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
