The Phospho-MAP2K2 (T394) Polyclonal Antibody (CABP0121) is a valuable tool for researchers studying the MAP2K2 protein, also known as MEK2. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.MAP2K2 is a key protein in the MAPK signaling pathway, playing a crucial role in cell proliferation, differentiation, and apoptosis. Phosphorylation of MAP2K2 at Threonine 394 is known to regulate its activity, making this antibody particularly useful for studying the activation of MAP2K2 in various cellular processes.
By enabling specific detection and analysis of phosphorylated MAP2K2, this antibody is ideal for investigations in cell biology, signal transduction, and cancer research. Understanding the regulation of MAP2K2 phosphorylation can provide insights into how this pathway contributes to diseases like cancer, making this antibody a valuable asset for researchers exploring therapeutic interventions targeting the MAPK pathway.
Product Name:
Phospho-MEK2-T394 Rabbit Polyclonal Antibody
SKU:
CABP0121
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A phospho specific peptide corresponding to residues surrounding T394 of human MEK2
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
5605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells using Phospho-MEK2-T394 Rabbit pAb (CABP0121).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
