The Phospho-MAP2K5 (Ser311/Thr315) Antibody (PAC023912) is a valuable tool for researchers studying the MAP2K5 protein. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in Western blot applications. By specifically binding to the phosphorylated form of MAP2K5 at Ser311/Thr315, this antibody enables precise detection and analysis of MAP2K5 in various cell types.MAP2K5, also known as dual specificity kinase, plays a crucial role in signaling pathways that regulate cell growth, differentiation, and response to stress.
Phosphorylation of MAP2K5 at Ser311/Thr315 is known to modulate its activity, making it an important target for research in cancer biology, neurology, and cardiovascular diseases. Understanding the role of phosphorylated MAP2K5 in cellular signaling pathways is essential for developing targeted therapies for diseases associated with dysregulated MAP2K5 activity.
Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5.
Form:
Liquid
Storage Buffer:
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Rat and Mouse brain tissue using MEK5(phospho-Ser311/ Thr315) Antibody.
Background:
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
Synonyms:
Map2k5
UniProt Protein Function:
MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 familyCellular Component: membrane; spindle; intracellular; nucleus; cytosolMolecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activityBiological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation
UniProt Protein Details:
NCBI Summary:
kinase involved in signal transduction in the cell [RGD, Feb 2006]
