Description
Phospho-MAP3K5-T918 Antibody (CABP0061)
The Phospho-MAP3K5-T918 Antibody (CABP0061) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of MAP3K5 at threonine 918, this antibody allows for the detection and analysis of MAP3K5 activation in various cell types.MAP3K5 is a crucial component of signaling cascades involved in cell growth, differentiation, and apoptosis. It is known to play a role in various biological processes, including immune response regulation and development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | Phospho-MAP3K5-T918 Antibody |
| SKU: | CABP0061 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | TETF T | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | ASK1, MEKK5, MAPKKK5, Phospho-MAP3K5-T918 |
| Positive Sample: | HeLa |
| Cellular Localization: | Cytoplasm, Endoplasmic Reticulum. |
| Calculated MW: | 155kDa |
| Observed MW: | 130kDa |
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
| Purification Method | Affinity purification |
| Gene ID | 4217 |
| RRID | AB_2771303 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from HeLa cells, using Phospho-MAP3K5-T918 Rabbit pAb (CABP0061) at 1:1000 dilution. HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. |
