The NACA Antibody (CAB10122) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blot analysis. By binding specifically to the NACA protein, this antibody allows for the accurate detection and analysis of NACA in different cell types.NACA, also known as nascent polypeptide-associated complex alpha subunit, plays a crucial role in maintaining the structure and function of chromatin, making it essential for gene regulation and DNA replication processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NACA Antibody
SKU:
CAB10122
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HSD48, NACA1, skNAC, NAC-alpha, NACA
Positive Sample:
293T, K-562, Mouse brain, Rat thymus
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
23kDa
Observed MW:
37kDa
This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.
Purification Method
Affinity purification
Gene ID
4666
RRID
AB_2757647
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using NACA Rabbit pAb (CAB10122) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
