The Phospho-NF-kB p65/RelA-S536 Monoclonal Antibody (CABP1294) is a high-quality antibody developed for reliable detection and analysis of target proteins. NF-kB is a transcription factor that plays a crucial role in regulating the expression of genes involved in immune responses, inflammation, cell proliferation, and survival. Phosphorylation of NF-kB p65 at serine 536 is known to activate its transcriptional activity and is implicated in various disease states, including cancer, inflammatory disorders, and autoimmune diseases.This monoclonal antibody, raised in mice, is highly specific for the phosphorylated form of NF-kB p65 (RELA) at serine 536 and demonstrates high sensitivity and specificity in Western blot applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-NF-kB p65/RelA-S536 Monoclonal Antibody
SKU:
CABP1294
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC55585
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FSSI A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:10000 - 1:40000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with TNF-α and Calyculin A, C6 treated with Calyculin A, NIH/3T3 treated with Calyculin A
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
60kDa
Observed MW:
65kDa
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5970
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (CABP1294) at 1:19000 dilution incubated at room temperature for 1.5 hours. HeLa cells were treated with TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Western blot analysis of lysates from C6 cells using Phospho-NF-kB p65/RelA-S536 Rabbit mAb (CABP1294) at 1:19000 dilution incubated at room temperature for 1.5 hours. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
