The Phospho-NF-kB p65/RelA-T505 Polyclonal Antibody (CABP1318) is a high-quality antibody developed for reliable detection and analysis of target proteins. NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 5970 Swiss Prot Synonym p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-T505
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-NF-kB p65/RelA-T505 Polyclonal Antibody
SKU:
CABP1318
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. RRID Gene ID 5970 Swiss Prot Synonym p65; CMCU; NFKB3; AIF3BL3; Phospho-NF-kB p65/RelA-T505
Purification Method:
Affinity purification
Gene ID:
5970
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U-2 OS cells, using Phospho-NF-kB p65/RelA-T505 Rabbit pAb (CABP1318) at 1:1000 dilution. U-2 OS cells were treated with cisplatin at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
