The Phospho-p53 (S46) Polyclonal Antibody (CABP0476) is a valuable tool for researchers studying the phosphorylation of the p53 protein at serine 46, a post-translational modification that is known to regulate the activity of p53 in response to DNA damage and cellular stress. This antibody, raised in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.The phosphorylation of p53 at serine 46 is a critical event in the activation of p53-mediated cell cycle arrest and apoptosis in response to genotoxic stress.
Dysregulation of this pathway has been linked to a variety of human cancers, making the study of phospho-p53 (S46) an important area of research in oncology.By enabling the specific detection and analysis of phospho-p53 (S46) in various cell types, the CABP0476 antibody offers researchers a powerful tool for investigating the role of this phosphorylation event in cancer progression and response to therapy. Its high specificity and sensitivity make it an ideal choice for studies in cancer biology, molecular oncology, and drug development.
Product Name:
Phospho-p53-S46 Rabbit Polyclonal Antibody
SKU:
CABP0476
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S46 of human p53 (NP_000537.3).
Sequence:
MLSP D
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Purification Method:
Affinity purification
Gene ID:
7157
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-p53-S46 Rabbit pAb (CABP0476) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
