The Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody (CABP0482) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at serine 371 of p70S6K1 in human samples and is validated for use in Western blot applications.Phosphorylation of p70S6K1 at serine 371 plays a crucial role in the activation of this protein, leading to downstream signaling that promotes cell proliferation and survival. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways that regulate cellular processes such as growth and metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-p70 S6 Kinase 1-S371 Polyclonal Antibody
SKU:
CABP0482
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VDSP D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method
Affinity purification
Gene ID
6198
RRID
AB_2771383
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-S371 Rabbit pAb (CABP0482) at 1:400 dilution. NIH/3T3 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
