The Phospho-PFKFB2-S483 Antibody (CABP0784) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. RRID AB_2771414 Gene ID 5208 Swiss Prot Synonym PFK-2/FBPase-2; Phospho-PFKFB2-S483
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PFKFB2-S483 Antibody
SKU:
CABP0784
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PFK-2/FBPase-2, Phospho-PFKFB2-S483
Positive Sample:
HeLa treated with IGF-1
Cellular Localization:
Cytosol, Nucleoplasm.
Calculated MW:
58kDa
Observed MW:
55kDa
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. RRID AB_2771414 Gene ID 5208 Swiss Prot Synonym PFK-2/FBPase-2; Phospho-PFKFB2-S483
Purification Method:
Affinity purification
Gene ID:
5208
RRID:
AB_2771414
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PFKFB2-S483 Rabbit pAb (CABP0784) at 1:2000 dilution. HeLa cells were treated with IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
