The Phospho-PIK3R1/PIK3R2/PIK3R3-Y467 Polyclonal Antibody (CABP0427) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] RRID AB_2771417 Gene ID 5295 5296 8503 Swiss Prot Synonym PIK3R1/PIK3R2/PIK3R3; Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199
This antibody is validated for use in WB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Cell-Cell Junction, Cis-Golgi Network, Cytoplasm, Cytosol, Nucleus, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
42-54kDa/81-84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] RRID AB_2771417 Gene ID 5295 5296 8503 Swiss Prot Synonym PIK3R1/PIK3R2/PIK3R3; Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199
Purification Method:
Affinity purification
Gene ID:
5295 5296 8503
RRID:
AB_2771417
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293 cells, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb (CABP0427). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
