The PI3 Kinase p85 beta Antibody (CAB17433) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, is highly specific to human samples and has been validated for Western blot and immunohistochemistry applications.PIK3R2 plays a crucial role in the PI3K signaling pathway, which is often dysregulated in cancer and other diseases. By targeting PIK3R2 with this antibody, researchers can study the role of this protein in cell signaling pathways, potentially leading to new insights into disease mechanisms and therapeutic targets.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PI3 Kinase p85 beta Antibody
SKU:
CAB17433
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene.
Purification Method
Affinity purification
Gene ID
5296
RRID
AB_2771664
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PI3 Kinase p85 beta Rabbit pAb (CAB17433) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using PI3 Kinase p85 beta Rabbit pAb (CAB17433) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta using PI3 Kinase p85 beta Rabbit pAb (CAB17433) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 using PI3 Kinase p85 beta Rabbit pAb (CAB17433) at dilution of 1:100. Blue: DAPI for nuclear staining.
