The Phospho-PKC alpha-T638 + PKC beta II-T1281 Monoclonal Antibody (CABP1375) is a high-quality antibody developed for reliable detection and analysis of target proteins. PKC enzymes play a vital role in regulating various cellular processes, including cell growth, differentiation, and survival. This antibody, developed through monoclonal antibody technology, specifically recognizes the phosphorylated forms of PKC Alpha at threonine 638 and PKC Beta II at threonine 1281.Validated for use in Western blot applications, this antibody allows for the detection and analysis of phosphorylated PKC Alpha and PKC Beta II proteins in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Purification Method
Affinity purification
Gene ID
5578 5579
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-PKC alpha-T638 + PKC beta II-T641 Rabbit mAb (CABP1375) at 1:6000 dilution.293T cells were treated by CIP(20μL/400μL) at 37℃ for 1 hour.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
