The Phospho-PKC zeta-T560 Monoclonal Antibody (CABP1149) is a high-quality antibody developed for reliable detection and analysis of target proteins. This post-translational modification is known to play a crucial role in the activation and function of PKC-zeta, a member of the protein kinase C family involved in various cellular processes such as cell proliferation, differentiation, and apoptosis.This antibody, developed using rabbit monoclonal technology, offers exquisite specificity and sensitivity for detecting phosphorylated PKC-zeta (T560) in human samples. It is validated for use in Western blotting applications, enabling accurate and reliable detection of the phosphorylated form of PKC-zeta in various cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Phospho-PKC zeta-T560 Monoclonal Antibody
SKU:
CABP1149
Size:
20μL, 100μL
Reactivity:
Rat
Clone Number:
ARC1617
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:3000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PKC2, PKC-ZETA, Phospho-PKC zeta-T560
Positive Sample:
C6
Cellular Localization:
Cell Junction, Cytoplasm, Endosome.
Calculated MW:
68kDa
Observed MW:
76kDa
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
5590
RRID
AB_2864013
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-PKC zeta-T560 Rabbit mAb (CABP1149) at 1:1000 dilution. Both 293T cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
