The Phospho-PKCdelta/theta-S643/676 Antibody (CABP0567) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PKCδ at the theta S643/676 site in human samples and is validated for use in Western blot applications. By binding to the phosphorylated PKCδ protein, this antibody allows for the detection and analysis of PKCδ activation in various cell types.PKCδ is a crucial player in diverse cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PKCδ at the theta S643/676 site is known to regulate its activity and function in signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-PKCdelta/theta-S643/676 Antibody
SKU:
CABP0567
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RLSY S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
5580 5588
RRID
AB_2771425
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from RAW264.7 cells, using Phospho-PKCδ/θ-S643/676 Rabbit pAb (CABP0567) at 1:1000 dilution. RAW 264.7 cells were treated with PMA (200 nM) for 30 minutes . Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
