The Phospho-PRKCQ-T538 Antibody (CABP0798) is a high-quality antibody developed for reliable detection and analysis of target proteins. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. RRID AB_2771461 Gene ID 5588 Swiss Prot Synonym PRKCT; nPKC-theta; Phospho-PRKCQ-T538
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PRKCQ-T538 Antibody
SKU:
CABP0798
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PRKCT, nPKC-theta, Phospho-PRKCQ-T538
Positive Sample:
Jurkat treated with PMA, HeLa treated with serum-starvation, HeLa treated with PMA
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. RRID AB_2771461 Gene ID 5588 Swiss Prot Synonym PRKCT; nPKC-theta; Phospho-PRKCQ-T538
Purification Method:
Affinity purification
Gene ID:
5588
RRID:
AB_2771461
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (CABP0798) at 1:2000 dilution. Jurkat cells were treated with PMA/TPA (200nM) for 10 minutes.HeLa cells were treated with serum-starvation overnight or treated with PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
