The Phospho-RB-S807/811 Antibody (CABP0484) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the phosphorylated form of the Retinoblastoma protein (Rb) at serine 807 and 811 residues. It is highly reactive with human samples and has been validated for use in Western blot applications.Rb is a key tumor suppressor protein that plays a crucial role in controlling cell proliferation and differentiation. Phosphorylation of Rb at serine 807 and 811 regulates its activity, allowing for progression through the cell cycle.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-RB-S807/811 Antibody
SKU:
CABP0484
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SPLK S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method
Affinity purification
Gene ID
5925
RRID
AB_2771496
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-RB-S807/811 Rabbit pAb (CABP0484) at 1:1000 dilution. HT-29 cells were treated with Nocodazole (100ng/mL) for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
