The Phospho-RB-S608 Antibody (CABP0570) is a high-quality antibody developed for reliable detection and analysis of target proteins. Rb1 is a tumor suppressor protein that plays a crucial role in cell cycle regulation and is commonly dysregulated in cancer.This polyclonal antibody, raised in rabbits, is highly specific for detecting phosphorylation at serine 608 of Rb1 in human samples. It is validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated Rb1 in various cell types.The phosphorylation of Rb1 at serine 608 has been shown to regulate its activity and function in cell cycle control and tumor suppression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-RB-S608 Antibody
SKU:
CABP0570
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YLSP V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.
Purification Method
Affinity purification
Gene ID
5925
RRID
AB_2771498
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-RB-S608 Rabbit pAb (CABP0570) at 1:2000 dilution. Jurkat cells were treated with 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
