The Phospho-NF-kB p65/RelA-S529 Antibody (CABP0944) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, also raised in rabbits, is highly specific to human samples and is optimized for use in Western blotting applications. By targeting the phosphorylated form of RelA, this antibody allows for the precise detection and analysis of RelA activation in various cell types.RelA is a key player in the NF-kB signaling pathway, which controls many aspects of immune and inflammatory responses. The phosphorylation of RelA at serine 529 is known to regulate its transcriptional activity and impact gene expression related to inflammation and cell survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-NF-kB p65/RelA-S529 Antibody
SKU:
CABP0944
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LLSG D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5970
RRID
AB_2863855
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells using Phospho-NF-kB p65/RelA-S529 Rabbit pAb (CABP0944) at 1:500 dilution incubated overnight at 4℃. HeLa cells were treated with TNF-α (100 ng/ml) and Calyculin A (50 nM) at 37℃ for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
