Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Antibody (CABP0538)
The Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Antibody (CABP0538) is a high-quality antibody developed for reliable detection and analysis of target proteins. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Antibody
SKU:
CABP0538
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
S6, eS6, Phospho-S6 Ribosomal Protein (RPS6)-S235/236
Positive Sample:
HeLa treated with 10% FBS, NIH/3T3 treated with 10% FBS, NIH/3T3 treated with PDGF, PC-12 treated with 10% FBS
Cellular Localization:
Cytoplasm, Cytoplasmic Ribonucleoprotein Granule, Cytosol, Cytosolic Ribosome, Endoplasmic Reticulum, Nucleolus, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm.
Calculated MW:
29kDa
Observed MW:
32kDa
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Purification Method
Affinity purification
Gene ID
6194
RRID
AB_2771522
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 Rabbit pAb (CABP0538) at 1:1000 dilution. HeLa cells NIH/3T3 cells and PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 1s.
