The Phospho-RPS6KA5-S376 Antibody (CABP0800) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-RPS6KA5-S376 Antibody
SKU:
CABP0800
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MSK1, RLPK, MSPK1, Phospho-RPS6KA5-S376
Positive Sample:
NIH/3T3 treated with Anisomycin
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
90kDa
Observed MW:
90kDa
Enables ATP binding activity and protein serine/threonine kinase activity. Involved in several processes, including histone-serine phosphorylation; positive regulation of histone modification; and regulation of transcription, DNA-templated. Located in cytoplasm and nucleoplasm.
Purification Method
Affinity purification
Gene ID
9252
RRID
AB_2771526
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-RPS6KA5-S376 Rabbit pAb (CABP0800) at 1:2000 dilution. NIH/3T3 cells were treated with Anisomycin (25μg/mL) for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using Phospho-RPS6KA5-S376 Rabbit pAb (CABP0800) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Phospho-RPS6KA5-S376 Rabbit pAb (CABP0800) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
