The SMAD3 Polyclonal Antibody (CAB11388) is a valuable tool for researchers studying SMAD3, a key protein involved in the TGF-beta signaling pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for Western blot applications. By specifically binding to the SMAD3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in molecular biology, cell signaling, and cancer research.SMAD3 is a crucial mediator of TGF-beta signaling, playing a vital role in regulating cell growth, differentiation, and apoptosis.
Dysregulation of the TGF-beta/SMAD3 pathway has been implicated in various diseases, including cancer, fibrosis, and immune disorders. Therefore, understanding the function of SMAD3 is essential for unraveling the mechanisms underlying these pathologies and developing targeted therapies.The SMAD3 Polyclonal Antibody (CAB11388) provides researchers with a reliable tool to study the expression and activity of SMAD3 in cellular processes and disease states. Its versatility and specificity make it an invaluable asset for investigations that aim to elucidate the role of SMAD3 in health and disease.
Product Name:
Smad3 Rabbit Polyclonal Antibody
SKU:
CAB11388
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 190-221 of human Smad3 (NP_005893.1).
The SMAD family of proteins are a group of intracellular signal transducer proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. The SMAD3 protein functions in the transforming growth factor-beta signaling pathway, and transmits signals from the cell surface to the nucleus, regulating gene activity and cell proliferation. This protein forms a complex with other SMAD proteins and binds DNA, functioning both as a transcription factor and tumor suppressor. Mutations in this gene are associated with aneurysms-osteoarthritis syndrome and Loeys-Dietz Syndrome 3.
Purification Method:
Affinity purification
Gene ID:
4088
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Smad3 antibody (CAB11388) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
