The Phospho-VASP-S157 Monoclonal Antibody (CABP1511) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using hybridoma technology, exhibits high specificity and sensitivity towards phosphorylated VASP at serine 157. It is validated for use in various applications, including Western blot, immunofluorescence, and immunohistochemistry.VASP, also known as vasodilator-stimulated phosphoprotein, plays a key role in cell motility, adhesion, and cytoskeletal organization. Phosphorylation of VASP at serine 157 is a critical post-translational modification that regulates its functions in cell signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-VASP-S157 Monoclonal Antibody
SKU:
CABP1511
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC64201
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RRVS NA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VASP, Phospho-VASP-S157
Positive Sample:
HEL, HEL treated with Forskolin
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
40kDa
Observed MW:
50kDa
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method
Affinity purification
Gene ID
7408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit mAb (CABP1511) at 1:3000 dilution. HEL cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
