The Phospho-VASP-S239 Antibody (CABP0187) is a high-quality antibody developed for reliable detection and analysis of target proteins. VASP is a key regulator of actin dynamics and cell migration, making it important for processes such as cell adhesion, spreading, and motility.This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated VASP at serine 239 in human samples. It has been rigorously validated for use in Western blot applications, allowing for precise and reliable analysis of phospho-VASP levels in various cell types and tissues.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-VASP-S239 Antibody
SKU:
CABP0187
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KVSK Q
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method
Affinity purification
Gene ID
7408
RRID
AB_2771640
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (CABP0187) at 1:1000 dilution. C6 cells were treated with Forskolin (10 uM) at 37℃ for 1 hour after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using Phospho-VASP-S239 Rabbit pAb (CABP0187) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
