The PIAS2 Antibody (CAB5654) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications, enabling detection and analysis of PIAS2 in various cell types.PIAS2, also known as E3 SUMO-protein ligase PIAS2, is a key regulator of immune responses and has been implicated in various diseases including cancer and autoimmune disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PIAS2 Antibody
SKU:
CAB5654
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DIP, MIZ1, SIZ2, ARIP3, PIASX, ZMIZ4, PIAS2
Positive Sample:
Mouse testis
Cellular Localization:
Nucleus, Nucleus Speckle, Pml Body.
Calculated MW:
68kDa
Observed MW:
75kDa
This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.
Purification Method
Affinity purification
Gene ID
9063
RRID
AB_2766414
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse testis, using PIAS2 Rabbit pAb (CAB5654) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using PIAS2 Rabbit pAb (CAB5654) at dilution of 1:20 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
