The PIAS4 Antibody (CAB5322) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables SUMO ligase activity and ubiquitin protein ligase binding activity. Involved in negative regulation of transcription, DNA-templated; positive regulation of protein sumoylation; and protein sumoylation. Located in cytoplasm and nucleus. RRID AB_2766134 Gene ID 51588 Swiss Prot Synonym PIASY; Piasg; ZMIZ6; PIAS-gamma; PIAS4
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PIAS4 Antibody
SKU:
CAB5322
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIASY, Piasg, ZMIZ6, PIAS-gamma, PIAS4
Positive Sample:
HeLa, 293T
Cellular Localization:
Nucleus, Pml Body.
Calculated MW:
57kDa
Observed MW:
57-75kDa
Enables SUMO ligase activity and ubiquitin protein ligase binding activity. Involved in negative regulation of transcription, DNA-templated; positive regulation of protein sumoylation; and protein sumoylation. Located in cytoplasm and nucleus. RRID AB_2766134 Gene ID 51588 Swiss Prot Synonym PIASY; Piasg; ZMIZ6; PIAS-gamma; PIAS4
Purification Method:
Affinity purification
Gene ID:
51588
RRID:
AB_2766134
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PIAS4 Rabbit pAb (CAB5322) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
