The PIAS4 Antibody (CAB5322) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting PIAS4 in human samples, making it an excellent choice for Western blot experiments.PIAS4, or Protein Inhibitor of Activated STAT4, is a key player in the regulation of gene expression and protein modification, impacting a diverse array of cellular processes. By targeting PIAS4 with this antibody, researchers can gain insights into how this protein influences immune cell function, inflammation, and potentially cancer development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PIAS4 Antibody
SKU:
CAB5322
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIASY, Piasg, ZMIZ6, PIAS-gamma, PIAS4
Positive Sample:
HeLa, 293T
Cellular Localization:
Nucleus, Pml Body.
Calculated MW:
57kDa
Observed MW:
57-75kDa
Enables SUMO ligase activity and ubiquitin protein ligase binding activity. Involved in negative regulation of transcription, DNA-templated; positive regulation of protein sumoylation; and protein sumoylation. Located in cytoplasm and nucleus.
Purification Method
Affinity purification
Gene ID
51588
RRID
AB_2766134
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using PIAS4 Rabbit pAb (CAB5322) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
