The PIGH Antibody (PACO22499) is a polyclonal antibody specifically designed for research involving the PIGH protein. This protein is essential for the assembly of the GPI (glycosylphosphatidylinositol) anchor, which attaches certain proteins to the cell membrane. The antibody, raised in rabbits, is highly reactive with samples from various species and is validated for use in Western blot and immunohistochemistry applications.The PIGH antibody binds specifically to the PIGH protein, allowing for its detection and analysis in different cell types. This makes it an ideal tool for studying GPI anchor biosynthesis and its role in various cellular processes.
Understanding the function of PIGH is crucial for research in areas such as cell biology, immunology, and genetic disorders related to GPI anchor deficiencies.Overall, the PIGH Antibody (PACO22499) is a valuable tool for researchers looking to dive deeper into the mechanisms of GPI anchor biosynthesis and its implications in various biological processes. Its specificity and reliability make it a key component in studies aimed at understanding the role of the PIGH protein in health and disease.
Antibody Name:
PIGH Antibody (PACO22499)
Antibody SKU:
PACO22499
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from C-terminal of human PIGH.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human brain tissue using PIGH antibody.
Background:
Part of the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.
Synonyms:
Phosphatidylinositol N-acetylglucosaminyltransferase subunit H; Phosphatidylinositol-glycan biosynthesis class H protein; PIG-H;
UniProt Protein Function:
PIGH: Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. Belongs to the PIGH family.Protein type: Transferase; Endoplasmic reticulum; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; EC 2.4.1.198Chromosomal Location of Human Ortholog: 14q24.1Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complexMolecular Function: catalytic activityBiological Process: GPI anchor biosynthetic process; preassembly of GPI anchor in ER membrane; protein modification process
UniProt Protein Details:
NCBI Summary:
This gene encodes an endoplasmic reticulum associated protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. The protein encoded by this gene is a subunit of the GPI N-acetylglucosaminyl (GlcNAc) transferase that transfers GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. [provided by RefSeq, Jul 2008]
