The PIK3CA Antibody (CAB0265) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PIK3CA protein, researchers can easily detect and analyze its expression in various cell types.PIK3CA is known to play a crucial role in cell growth, survival, and proliferation, making it a key player in cancer development and progression. Mutations in the PIK3CA gene have been linked to various types of cancer, making it a promising target for cancer therapy research.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PIK3CA Antibody
SKU:
CAB0265
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cytoplasm, Cytosol, Intercalated Disc, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
124kDa
Observed MW:
110kDa
Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Purification Method
Affinity purification
Gene ID
5290
RRID
AB_2757078
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PIK3CA Rabbit pAb (CAB0265) at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PIK3CA Rabbit pAb (CAB0265) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using PIK3CA Rabbit pAb (CAB0265) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PIK3CA Rabbit pAb (CAB0265) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using PIK3CA Rabbit pAb (CAB0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using PIK3CA Rabbit pAb (CAB0265) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
