The PIM1 Polyclonal Antibody (CAB24475) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By targeting the PIM1 protein, researchers can accurately detect and analyze its expression in various cell types, providing valuable insights into its role in cancer and other diseases.The PIM1 protein is a serine/threonine kinase that is frequently overexpressed in various cancers, including lymphoma, leukemia, and solid tumors. Its aberrant activity has been linked to increased cell proliferation, survival, and resistance to chemotherapy, making it an attractive target for drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PIM1 Polyclonal Antibody
SKU:
CAB24475
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIM1, PIM, serine/threonine-protein kinase pim-1
Positive Sample:
Rat kidney
Cellular Localization:
Cell Membrane, Cytoplasm, Nucleus.
Calculated MW:
35kDa/45kDa
Observed MW:
44kDa
The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).
Purification Method
Affinity purification
Gene ID
5292
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat kidney, using PIM1 Rabbit pAb (CAB24475) at 1:600 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
