The PITPNA Antibody (CAB12966) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting. By binding specifically to PITPNA, this antibody enables precise detection and analysis of the protein in different cell types, making it ideal for studies in cell biology, lipid signaling, and membrane trafficking.PITPNA, also known as phosphatidylinositol transfer protein alpha, is crucial for the transport of phosphatidylinositol, a key component of cell membranes, and is involved in regulating important cellular processes such as signal transduction and vesicular trafficking.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PITPNA Antibody
SKU:
CAB12966
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PITPN, VIB1A, HEL-S-36, PI-TPalpha, PITPNA
Positive Sample:
U-87MG, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat brain, Rat heart
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
35kDa
This gene encodes a member of a family of lipid-binding proteins that transfer molecules of phosphatidylinositol or phosphatidylcholine between membrane surfaces. The protein is implicated in phospholipase C signaling and in the production of phosphatidylinositol 3,4,5-trisphosphate (PIP3) by phosphoinositide-3-kinase.
Purification Method
Affinity purification
Gene ID
5306
RRID
AB_2759813
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PITPNA Rabbit pAb (CAB12966) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of L929 cells using PITPNA Rabbit pAb (CAB12966) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
