The PKC alpha Monoclonal Antibody (CAB23231) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed using advanced technology, specifically targets PKC-alpha and is highly reactive with human samples.PKC-alpha is a critical player in cellular processes such as cell growth, differentiation, and apoptosis. Dysregulation of PKC-alpha has been linked to various diseases, including cancer, neurological disorders, and cardiovascular diseases. By using this monoclonal antibody in experiments such as Western blotting, researchers can effectively detect and analyze PKC-alpha levels in different cell types.
This antibody is validated for use in IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PKC alpha Monoclonal Antibody
SKU:
CAB23231
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58845
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
IF/ICCELISA
Recommended Dilution:
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method
Affinity purification
Gene ID
5578
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Immunofluorescence analysis of HeLa cells using PKC alpha Rabbit mAb (CAB23231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
