The PKIA Antibody (PAC023239) is a polyclonal antibody designed for research involving protein kinase inhibitor alpha (PKIA), a key player in regulating signal transduction pathways involved in various cellular processes. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications.PKIA is known to inhibit the activity of protein kinase A (PKA), a crucial enzyme involved in cell growth, proliferation, and differentiation. By targeting PKIA, researchers can gain insights into the regulatory mechanisms of PKA and its role in various physiological and pathological conditions.
This antibody enables the detection and analysis of PKIA in different cell types, providing valuable information for studies in cell signaling, cancer research, and drug development.Understanding the functions and interactions of PKIA can lead to novel therapeutic strategies for targeting specific signaling pathways that are dysregulated in diseases such as cancer, cardiovascular disorders, and neurological conditions. The PKIA Antibody is a valuable tool for researchers seeking to elucidate the molecular mechanisms underlying these diseases and develop targeted treatments.
Synthesized peptide derived from internal of human IPKA.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TPD52 antibody.
Immunofluorescence analysis of HeLa cells, using TPD52 antibody.
Background:
Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
Synonyms:
IPKA; OKRBCI; PKI-alpha; PRKACN1; cAMP-dependent protein kinase inhibitor alpha
UniProt Protein Function:
PKIA: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains. Belongs to the PKI family.
UniProt Protein Details:
Protein type:Inhibitor
Chromosomal Location of Human Ortholog: 8q21.13
Cellular Component: cytoplasm; nucleus
Molecular Function:cAMP-dependent protein kinase inhibitor activity; protein binding
Biological Process: negative regulation of cAMP-dependent protein kinase activity; negative regulation of protein import into nucleus; negative regulation of transcription from RNA polymerase II promoter; regulation of G2/M transition of mitotic cell cycle
NCBI Summary:
The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
