The PKLR Antibody (CAB12084) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By binding specifically to the PKLR protein, this antibody enables researchers to detect and analyze PKLR expression in various cell types.PKLR is a key player in the glycolytic pathway, with mutations in the PKLR gene being associated with disorders such as pyruvate kinase deficiency and hemolytic anemia.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PKLR Antibody
SKU:
CAB12084
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PK1, PKL, RPK, PKRL, PKLR
Positive Sample:
Mouse heart, Rat kidney
Cellular Localization:
Cytoplasm, Cytosol, Extracellular Exosome.
Calculated MW:
62kDa
Observed MW:
62kDa
The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5313
RRID
AB_2758983
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PKLR Rabbit pAb (CAB12084) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 50s.
