The PRK2/PKN2 Antibody (CAB18373) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA polymerase binding activity; histone deacetylase binding activity; and protein serine/threonine kinase activity. Involved in several processes, including apical junction assembly; positive regulation of cell cycle; and positive regulation of viral genome replication. Located in several cellular components, including cleavage furrow; cytoskeleton; and midbody.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PRK2/PKN2 Antibody
SKU:
CAB18373
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Apical Junction Complex, Centrosome, Cytoplasm, Cytosol, Intermediate Filament Cytoskeleton, Nuclear Body, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
112kDa
Observed MW:
140kDa
Enables RNA polymerase binding activity; histone deacetylase binding activity; and protein serine/threonine kinase activity. Involved in several processes, including apical junction assembly; positive regulation of cell cycle; and positive regulation of viral genome replication. Located in several cellular components, including cleavage furrow; cytoskeleton; and midbody.
Purification Method
Affinity purification
Gene ID
5586
RRID
AB_2862140
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat lung, using PRK2/PRK2/PKN2 Rabbit pAb (CAB18373) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
