The PKR (EIF2AK2) Polyclonal Antibody is a versatile tool designed for researchers studying the protein kinase R (PKR), also known as EIF2AK2, involved in regulating immune responses and apoptosis. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PKR is a crucial regulator of the cellular stress response, playing a key role in antiviral defense, immune signaling, and cell death pathways. By detecting PKR in various cell types, this antibody allows for the investigation of its function in immune regulation, viral infections, and cancer development.
With its ability to bind to PKR, this antibody enables researchers to study the role of this protein in both physiological and pathological conditions, making it a valuable tool for those studying immunology, virology, and cancer biology. Gain insights into the complex functions of PKR with the PKR (EIF2AK2) Polyclonal Antibody.
Product Name:
PKR/EIF2AK2 Polyclonal Antibody
SKU:
CAB4047
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PKR/EIF2AK2 (NP_002750.1).
The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.
Purification Method:
Affinity purification
Gene ID:
5610
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using PKR/EIF2AK2 Rabbit pAb (CAB4047) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
