The PLA2R1 Monoclonal Antibody (CAB23148) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced through hybridoma technology, exhibits high specificity and sensitivity in detecting PLA2R1 in human samples. Validated for use in various applications including immunohistochemistry and ELISA, this antibody allows for precise and reliable detection of PLA2R1 in tissue samples, making it an invaluable asset for studies in nephrology and autoimmune disease research.PLA2R1, a receptor for secretory phospholipase A2, is a crucial component in the pathogenesis of membranous nephropathy, an autoimmune kidney disease characterized by the deposition of autoantibodies against PLA2R1 in the glomerular basement membrane.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PLA2R1 Monoclonal Antibody
SKU:
CAB23148
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC59749
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PLA2R, PLA2-R, PLA2IR, CLEC13C, PLA2G1R, PLA2R1
Positive Sample:
HeLa, Mouse brain, Rat liver
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
169kDa
Observed MW:
180kDa
This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
22925
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PLA2R1 Rabbit mAb (CAB23148) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
Western blot analysis of lysates from Rat liver using PLA2R1 Rabbit mAb (CAB23148) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
